Expressed sequence tag (EST): A unique stretch of DNA within a coding region of a gene that is useful for identifying full-length genes and serves as a landmark for mapping . An EST is a sequence tagged site ( STS ) derived from cDNA .

An STS is a short segment of DNA which occurs but once in the genome and whose location and base sequence are known. STSs are detectable by the polymerase chain reaction ( PCR ), are helpful in localizing and orienting mapping and sequence data, and serve as landmarks in the physical map of the genome.

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