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Sequence tagged site (STS): A short (200 to 500 base pair) DNA sequence that occurs but once in the genome and whose location and base sequence are known.

STSs are detectable by polymerase chain reaction ( PCR ), are useful for localizing and orienting the mapping and sequence data, and serve as landmarks on the physical map of a genome.

Expressed sequence tags (ESTs) are STSs derived from cDNAs (complementary DNAs).

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